Coding

Part:BBa_K2271014:Design

Designed by: Fiona Edenhofer   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


VioA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2222
    Illegal BglII site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promotor (ScTDH3; strong) and terminator (ScTDH1) are optimized for yeast, whereas the coding sequence is not. A 6X-His/3X-Flag-Tag is added to the N-terminus.

Source

VioA was amplificated via PCR from the biobrick VioABCE (Part:BBa_K274004).

References